1. Optical Coherence Tomography of Descemet Membrane Separation by the Big Bubble Technique

    Purpose: To image Descemet membrane separation by the big bubble technique in human corneas by using anterior segment optical coherence tomography (OCT). Methods: Five human corneoscleral rims were placed on an artificial anterior chamber and partially trephinated. A 23-gauge needle was inserted into the stroma under slit-lamp control and air was injected. The procedure was continuously imaged by anterior segment OCT. Results: In all corneoscleral rims, a big bubble was created. The spread of air seemed to follow the interlamellar spaces without crossing lamellae. It involved mainly the inner layers of the stroma while sparing the outer 212 +/- 41 [mu ...
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