Feature Of The Week 11/16/14: In Vivo Analysis of Burns in a Mouse Model using Spectroscopic OCT
Evaluation of burn severity is a significant clinical problem with over 2 million burns reported each year in the United States . The magnitude of this clinical need has inspired the development of many technologies to assess burn depth, but none of these have been widely adopted as clinical judgment still relies primarily on visual inspection, with an accuracy of about 70% .
A recent study by researchers at Duke University used spectroscopic optical coherence tomography (OCT) to evaluate burned animal tissues in vivo. Spectroscopic OCT is an extension of conventional OCT that can measure depth-resolved scattering and absorption spectra . Spectroscopic information is obtained by processing the backscattered signal with an appropriate time-frequency analysis such as a short-time Fourier transform or the recently developed dual window method . The study compared multiple processing and analysis methods and found that each approach showed clear spectral differences between healthy and burned tissues. For example, the burned tissue spectra showed oscillatory features that are similar to the absorption spectrum of adipose tissue. This observation was supported by the corresponding histopathology, which showed that the fat cells in the burned tissues were no longer confined beneath the dermis. These spectral differences were leveraged to classify the health of the tissue producing an accuracy of 91%. Overall, the results suggest that spectroscopic OCT could be a useful tool for studying the nature and extent of burn injuries.
For more information see references below and recent Article. Courtesy Jason Maher and Adam Wax from Duke University.
1. D. S. Kauvar, S. E. Wolf, C. E. Wade, L. C. Cancio, E. M. Renz, and J. B. Holcomb, "Burns sustained in combat explosions in Operations Iraqi and Enduring Freedom (OIF/OEF explosion burns)," Burns : journal of the International Society for Burn Injuries 32, 853-857 (2006).
2. A. D. Jaskille, J. C. Ramella-Roman, J. W. Shupp, M. H. Jordan, and J. C. Jeng, "Critical review of burn depth assessment techniques: part II. Review of laser doppler technology," J Burn Care Res 31, 151-157 (2010).
3. U. Morgner, W. Drexler, F. X. Kärtner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, "Spectroscopic optical coherence tomography," Opt. Lett. 25, 111-113 (2000).
4. F. Robles, R. N. Graf, and A. Wax, "Dual window method for processing spectroscopic optical coherence tomography signals with simultaneously high spectral and temporal resolution," Opt Express 17, 6799-6812 (2009).