1. Hybrid single-source online Fourier transform coherent anti-Stokes Raman scattering/optical coherence tomography

    Hybrid single-source online Fourier transform coherent anti-Stokes Raman scattering/optical coherence tomography

    We demonstrate a multimodal optical coherence tomography (OCT) and online Fourier transform coherent anti-Stokes Raman scattering (FTCARS) platform using a single sub-12 femtosecond (fs) Ti:sapphire laser enabling simultaneous extraction of structural and chemical (“morphomolecular”) information of biological samples. Spectral domain OCT prescreens the specimen providing a fast ultrahigh ( 4 × 12     μ m axial and transverse) resolution wide field morphologic overview. Additional complementary intrinsic molecular information is obtained by zooming into regions of interest for fast label-free chemical mapping with online FTCARS spectroscopy. Background-free CARS is based on a Michelson interferometer in combination with a highly linear piezo stage, which allows ...

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