Feature Of The Week 6/16/13: Using Adaptive Optics Optical Coherence Tomography for in vivo Mouse Retinal Imaging
Small animal models of human diseases serve as a vital component in modern medical research, including eye-related research, where they facilitate the understanding of the underlying biological processes of human disorders, and development of novel therapies against vision-robbing diseases.
Non-invasive ophthalmic imaging modalities such as optical coherence tomography have become an important tool for small animal vision research programs and has greatly accelerated numerous preclinical studies. OCT offers the ability to perform longitudinal in vivo studies which are not feasible with more invasive imaging techniques such as immunohistology. However, to better visualize the retinal cellular microstructure and understand the molecular processes in living small animal, high-resolution retinal imaging is desired.
The goal of this project is to investigate the strategies to overcome the difficulties in high-resolution mouse retinal imaging and study the effects of increased lateral resolution and acquisition speed on visualizing mouse retina in vivo with OCT. In order to increase the lateral resolution, we incorporated adaptive optics into the sample arm of our custom mouse OCT system that allows correction of aberrations in the imaging path, including the optics of the eye. Additionally, a commercially available refraction cancelling lens was used to reduce lower order aberrations and specular back-reflection from the cornea. The performance of the AO system for correcting residual wavefront aberration in the mice eyes is presented. Results of AO FD-OCT images of mouse retina acquired in vivo with and without AO correction are also shown.
For more information see recent Article. Courtesy of Yifan Jian from Simon Fraser University.