Stimulus-Specific Pupil Dynamics Measured in Birds (Gallus gallus domesticus) In Vivo with Ultrahigh Resolution Optical Coherence Tomography
Purpose. To demonstrate the ability of high speed, ultrahigh-resolution optical coherence tomography (UHR-OCT) to measure and characterize in vivo visual stimulus-specific pupil dynamics in birds. Methods. Ten two-week old White Leghorn ( Gallus gallus domesticus ) chickens were imaged in this study. The chickens were dark-adapted for 1 hour and anesthetized with 2% isoflurane prior to the imaging procedure. Blue, green, and red single flash visual stimuli of 7 ms duration were used to evoke pupillary responses. UHR-OCT cross-sectional images of the pupil were acquired prior, during, and for several seconds after the visual stimuli onset. Images were processed with a novel ...
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