Imaging Mouse Embryonic Cardiovascular Development
arly development of the mammalian cardiovascular system is a highly dynamic process. Live imaging is an essential tool for analyzing normal and abnormal cardiovascular development and dynamics. This article describes two optical approaches for live dynamic imaging of mouse embryonic cardiovascular development: confocal microscopy and optical coherence tomography (OCT). Confocal microscopy, used in combination with fluorescent protein reporter lines, enables visualization of the developing and remodeling cardiovascular system with submicron resolution and even allows visualization of subcellular details of labeled structures. We describe mouse transgenic lines that can be used to image the developing vasculature and characterize hemodynamics by tracking ...
Login to comment.