Stimulus Specific Pupil Dynamics Measured in Birds (Gallus Gallus Domesticus) in vivo with Ultrahigh Resolution Optical Coherence Tomography
Purpose:To demonstrate the ability of high speed, ultra-high resolution optical coherence tomography (UHR-OCT) to measure and characterize in vivo visual stimulus specific pupil dynamics in birds. Methods:Ten two-week old White Leghorn (Gallus gallus domesticus) chickens were imaged in this study. The chickens were dark adapted for one hour and anesthetized with 2% isoflurane prior to the imaging procedure. Blue, green, and red single flash visual stimuli of 7ms duration were used to evoke pupillary responses. UHR-OCT cross-sectional images of the pupil were acquired prior, during and for several seconds after the visual stimuli onset. Images were processed with ...
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