1. Label-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography

    Label-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography

    Early patterning and polarity is of fundamental interest in preimplantation embryonic development. Label-free subcellular 3D live imaging is very helpful to its related studies. We have developed a novel system of full-field optical coherence tomography (FF-OCT) for noninvasive 3D subcellular live imaging of preimplantation mouse embryos with no need of dye labeling. 3D digitized embryos can be obtained by image processing. Label-free 3D live imaging is demonstrated for the mouse embryos at various typical preimplantation stages with a spatial resolution of 0.7 µm and imaging rate of 24 fps. Factors that relate to early patterning and polarity, such as ...

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