1. Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy

    Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy

    Optical coherence tomography (OCT), as a non-invasive technique for studying tissue morphology, is widely used in in vivo studies, requiring high resolution and fast three-dimensional imaging. Based on light scattering it reveals micrometer sized substructures of the samples due to changes in their optical properties and therefore allows quantification of the specimen's geometry. Utilizing fluorescence microscopy further information can be obtained from molecular compositions embedded in the investigated object. Fluorescent markers, specifically binding to the substance of interest, reveal the sample's chemical structure and give rise to functional studies. This research presents the application of a combined OCT ...

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