1. Ultrafast lasers advance deep-tissue imaging Since the first landmark two-photon microscopy images in 1990, a wide range of nonlinear imaging methods that utilize ultrafast lasers have evolved, including three-photon fluorescence excitation, second- and third-harmonic generation (THG), coherent anti-Stokes Raman spectroscopy (CARS), and other types of nonlinear optical effects.1 Two advantages of multiphoton microscopy are its inherent ability to provide high-resolution three-dimensional images at significant depths and to do this in live (Read Full Article)

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