1. Three-dimensional optical coherence tomography imaging of retinal sheet implants in live rats

    Three-dimensional optical coherence tomography imaging of retinal sheet implants in live rats
    Purpose To obtain three-dimensional images from retinal transplants in live animals and evaluate the placement and structural quality of the transplants. Methods Donor retinal sheets were isolated from E19 fetuses of transgenic rats expressing human alkaline phosphatase (hPAP), and transplanted to the subretinal space of 19–56 days old S334ter-3 rat recipients with fast retinal degeneration (average age at surgery 32 days). A total of 143 rats were imaged 1 day to 2.8 months after surgery, using a Fourier-domain optical coherence tomography (FDOCT) system, with an axial resolution of 3.5 μm. The CCD A-line integration time was set ...
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