1. Multimodality optical imaging combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) for morphological and biochemical tissue characterization

    Multimodality optical imaging combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) for morphological and biochemical tissue characterization

    Here we report on our current efforts to simultaneously quantify both morphological and biochemical tissue information by combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM). The Fourier domain OCT module is built around a custom designed high-speed spectrometer (bandwidth of 102 nm, 3 dB rolloff of 1.2 mm, lines rates of up to 59 kHz). A 40 nm bandwidth SLED centered at 830 nm provided an axial resolution of 7.6 mm for OCT. The objective lens provided 10 um lateral resolution for OCT and 100 um for FLIM. Lateral OCT and FLIM beam scanning was accomplished ...

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