Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering

Entities Mentioned

• Harvard University
• Massachusetts General Hospital
• Chulmin Joo
• Johannes F. de Boer
• VU University Amsterdam

1. Quantitative measurement of diffusive and directional processes of intracellular structures is not only critical in understanding cell mechanics and functions, but also has many applications, such as investigation of cellular responses to therapeutic agents. We introduce a label-free optical technique that allows non-perturbative characterization of localized intracellular dynamics. The method combines a field-based dynamic light scattering analysis with a confocal interferometric microscope to provide a statistical measure of the diffusive and directional motion of scattering structures inside a microscopic probe volume. To demonstrate the potential of this technique, we examined the localized intracellular dynamics in human epithelial ovarian cancer cells. ... (Read Full Article)

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