3-D intraoperative imaging combining an operating microscope with optical coherence tomography (OCT)

ScienceDirect May 8 2009 Microscopy , Otolaryngology

Objective Optical coherence tomography (OCT) allows non-contact/non-invasive analysis of tissues with a penetration depth of 2–3 mm reaching a spatial resolution of approximately 4–15 μm. This resolution is compatible with the resolution of modern operation microscopes. Aim of this work was to provide a three-dimensional (3D) intraoperative visualization of tissue structures. Material and methods A high-speed spectral domain OCT (a modified version of the Ganymed, Thorlabs HL) was adapted to a motorized operation microscope (HR 1000, Möller-Wedel). Via a specially designed two axis-scanner, the 840 nm OCT was adapted directly to a camera port of the operation ...

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