1. Inverse spectroscopic optical coherence tomography (ISOCT) for characterization of particle size and concentration

    Inverse spectroscopic optical coherence tomography (ISOCT) for characterization of particle size and concentration

    Inverse spectroscopic optical coherence tomography (IS-OCT) methods apply inverse problem formulations to acquired spectra to estimate depth-resolved sample properties. In the current study, we modelled the time-frequency-distributions using Lambert-Beer law and implemented IS-OCT using backscattering spectra calculated from Mie theory, then demonstrated the algorithm on polystyrene microspheres under idealized conditions. The results are significant because the method is compatible with high concentrations and broad diameter distributions of scattering particles such as organelles in biological cells.

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