1. Real-time assessment and characterization of immobilized lipase onto a natural matrix and qualitative reaction kinetic studies using swept-source optical coherence tomography

    Real-time assessment and characterization of immobilized lipase onto a natural matrix and qualitative reaction kinetic studies using swept-source optical coherence tomography

    The current study presents a method based on Optical Coherence Tomography (OCT) for non-destructive, real-time analysis and portrayal of immobilization efficacy for lipase on a natural matrix namely, eggshell. Subsequently, qualitative biochemical reaction kinetics of immobilized lipase was also studied. Successful immobilization of lipase on eggshell was confirmed by the presence of a clear peak in ‘A’ scan of OCT image. From immobilization kinetics it is clearly observed that the thickness of the highest peak of the A-scan increases significantly and peak intensity saturated after 90 min of incubation. Hydrolysis of oil using immobilized lipase indicated that the release of ...

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