1. Feature Of The Week

    Three-dimensional (3D) tumor spheroid models have gained increased recognition as important tools in cancer research and anti-cancer drug development. However, currently available imaging approaches employed in high-throughput screening drug discovery platforms e.g. bright field, phase contrast, and fluorescence microscopies, are unable to resolve 3D structures deep inside (>50 μm) tumor spheroids. In this study, we established a label-free, non-invasive optical coherence tomography (OCT) imaging platform to characterize 3D morphological and physiological information of multicellular tumor spheroids (MCTS) growing from ~250 μm up to ~600 μm in height over 21 days. In particular, tumor spheroids of two cell lines glioblastoma (U-87 MG) and colorectal carcinoma (HCT 116) exhibited distinctive evolutions in their geometric shapes at late growth stages. Volumes of MCTS were accurately quantified using a voxel-based approach without presumptions of their geometries. In contrast, conventional diameter-based volume calculations assuming perfect spherical shape resulted in large quantification errors. Furthermore, we successfully detected necrotic regions within these tumor spheroids based on increased intrinsic optical attenuation, suggesting a promising alternative of label-free viability tests in tumor spheroids. Therefore, OCT can serve as a promising imaging modality to characterize morphological and physiological features of MCTS, showing great potential for high-throughput drug screening.

    For more information see recent Article. Courtesy Chao Zhou from Lehigh University. To share this article click Here.
  2. Recent Articles From Around The Web

    1. OCT Made Easy (Textbook)

      OCT Made Easy (Textbook)

      This book, written by premier authors in the field of OCT intravascular imaging, covers the best practices for using OCT to provide high resolution cross-sectional viewing for atherosclerotic plaque assessment, stent strut coverage and apposition, assessment in stent restenosis evaluation, and PCI guide and optimization. Fully illustrated thorughout in a handy, cath-lab side handbook supplemented by online movie clips, OCT Made Easy includes case studies, angiography, CT correlations, and simple techniques for getting the best image.

      Read Full Article
    2. 3D Mapping of Choroidal Thickness from OCT B-Scans

      3D Mapping of Choroidal Thickness from OCT B-Scans

      The choroid is the middle layer of the eye globe located between the retina and the sclera. It is proven that choroidal thickness is a sign of multiple eye diseases. Optical Coherence Tomography (OCT) is an imaging technique that allows the visualization of tomographic images of near surface tissues like those in the eye globe. The automatic calculation of the choroidal thickness reduces the subjectivity of manual image analysis as well as the time of large scale measurements. In this paper, a method for the automatic estimation of the choroidal thickness from OCT images is presented. The pre-processing of the ...

      Read Full Article
    3. Multi-layer 3D Simultaneous Retinal OCT Layer Segmentation: Just-Enough Interaction for Routine Clinical Use

      Multi-layer 3D Simultaneous Retinal OCT Layer Segmentation: Just-Enough Interaction for Routine Clinical Use

      All current fully automated retinal layer segmentation methods fail in some subset of clinical 3D Optical Coherence Tomography (OCT) datasets, especially in the presence of appearance-modifying retinal diseases like Age-related Macular Degeneration (AMD), Diabetic Macular Edema (DME), and others. In the presence of local or regional failures, the only current remedy is to edit the obtained segmentation in a slice-by-slice manner. This is a very tedious and time-demanding process, which prevents the use of quantitative retinal image analysis in clinical setting. In turn, the non-existence of reliable retinal layer segmentation methods substantially limits the use of precision medicine concepts in ...

      Read Full Article
    4. Optical Coherence Tomography Denoising by Means of a Fourier Butterworth Filter-Based Approach

      Optical Coherence Tomography Denoising by Means of a Fourier Butterworth Filter-Based Approach

      Optical Coherence Tomography (OCT) is affected by ubiquitous speckle noise that difficult the visualization and analysis of the retinal structures. Any denoising strategy should be able to remove efficiently the noise as well as preserves clinical information contained in the images. This information is crucial to analyses the retinal layer tissue that allows the posterior analysis and recognition of relevant diseases as macular edema or diabetic retinopathy. To address this issue, a method based on the Fourier Butterworth filter combined with a contrast enhancement and a histogram regularization was developed in order to reduce the speckle noise in OCT retinal ...

      Read Full Article
    5. Feature Definition and Selection for Epiretinal Membrane Characterization in Optical Coherence Tomography Images

      Feature Definition and Selection for Epiretinal Membrane Characterization in Optical Coherence Tomography Images

      Optical Coherence Tomography (OCT) is a common imaging technique for the detection and analysis of optical diseases, since it is a non invasive method that generates in vivo a cross-sectional visualization of the retinal tissues. These characteristics contributed to the use of OCT imaging in the analysis of pathologies as, for instance, vitreomacular traction, age-related macular degeneration or hypertension. Among its applications, OCT imaging can be used in the detection of any present epiretinal membrane section in the retina, a critical issue to prevent further complications caused by this pathology. This work analyzed the main characteristics of the epiretinal membrane ...

      Read Full Article
    6. Resin infiltration analysis into carious lesions by optical coherence tomography

      Resin infiltration analysis into carious lesions by optical coherence tomography

      Purpose/aim: The minimally invasive approach claims for the detection and treatment of early caries lesions. Resin infiltrant materials can penetrate into porosities of the lesion body by capillary forces, preventing the progression of white spot lesions, improving esthetic and providing support to enamel. It is suggested that optical coherence tomography (OCT) could assess penetration depth of the resin infiltrant into white spots lesions, which may provide information about the lesion's progression.

      Read Full Article
    7. Hybrid Deep Learning on Single Wide-field Optical Coherence Tomography Scans Accurately Classifies Glaucoma Suspects

      Hybrid Deep Learning on Single Wide-field Optical Coherence Tomography Scans Accurately Classifies Glaucoma Suspects

      Purpose: Existing summary statistics based upon optical coherence tomographic (OCT) scans and/or visual fields (VFs) are suboptimal for distinguishing between healthy and glaucomatous eyes in the clinic. This study evaluates the extent to which a hybrid deep learning method (HDLM), combined with a single wide-field OCT protocol, can distinguish eyes previously classified as either healthy suspects or mild glaucoma. Methods: In total, 102 eyes from 102 patients, with or suspected open-angle glaucoma, had previously been classified by 2 glaucoma experts as either glaucomatous (57 eyes) or healthy/suspects (45 eyes). The HDLM had access only to information from a ...

      Read Full Article
    8. Avinger Announces First Patient Enrolled in its IDE Study of the Pantheris Image-Guided Atherectomy System for Treatment of In-Stent Restenosis

      Avinger Announces First Patient Enrolled in its IDE Study of the Pantheris Image-Guided Atherectomy System for Treatment of In-Stent Restenosis

      Avinger, Inc. (NASDAQ:AVGR), a leading developer of innovative treatments for Peripheral Artery Disease (PAD), today announced initiation of INSIGHT, a prospective, global, single arm, multi-center study to evaluate the safety and effectiveness of the Pantheris® Lumivascular Atherectomy System for treating in-stent restenosis (ISR) in lower extremity arteries. Up to 140 patients will be enrolled in the INSIGHT trial at up to 20 U.S. and international centers. Sean Janzer, M.D. of Einstein Medical Center and Glen Schwartzberg, M.D. of Baton Rouge General Medical Center serve as co-principal investigators of the study. In-stent restenosis occurs when a blocked ...

      Read Full Article
    9. The Role of Optical Coherence Tomography Angiography in Polypoidal Choroidal Vasculopathy

      The Role of Optical Coherence Tomography Angiography in Polypoidal Choroidal Vasculopathy

      Retinal imaging technology has progressed rapidly in recent years and plays increasingly vital roles in guiding the clinical management of retinal diseases, as well as facilitating greater understanding of the disease process. Polypoidal choroidal vasculopathy (PCV) is an important variant of neovascular age-related macular degeneration with distinct phenotypic features that can be seen both on clinical examination and using indocyanine green angiography (ICGA). In this issue of JAMA Ophthalmology, the optical coherence tomography angiography (OCTA) features of PCV are described and evaluated by Chi et al.1

      Read Full Article
    10. Development of a Free Space, LED Illuminated Spectral-domain Optical Coherence Tomography Setup

      Development of a Free Space, LED Illuminated Spectral-domain Optical Coherence Tomography Setup

      Free-space spectral domain optical coherence tomography has been demonstrated using an 8 mW ultra-bright 850 nm light-emitting diode with a 40 nm spectral width. The system detects longitudinal reflectivity of surface and sub-surface layers of optical elements to depths of a millimetre with high fidelity. Development stages included mathematical analysis of light interference by superposition of electric field phasors of reference and sample arms of a Michelson interferometer. A method by which depth-resolved reflectivity is acquired is described. A locally assembled Czerny Turner monochromator was aligned such that the interferometer output beam is dispersed into its spectral components before image ...

      Read Full Article
    11. Design and Implementation of Full Field-Optical Coherence Tomography on an Olympus IX73 Microscope (Thesis)

      Design and Implementation of Full Field-Optical Coherence Tomography on an Olympus IX73 Microscope (Thesis)

      Optical Coherence Tomography (OCT) has established itself as an important imaging technology for biomedical and industrial applications. In this thesis, we describe the design and implementation of a novel Full-Field OCT optical module that is specifically designed to fit inside an Olympus IX73 inverted microscope. We describe the design, components, and implementation of this novel optical module in detail. The resulting FF-OCT setup (microscope and OCT optical module) can be used as either a Time-Domain system or a Fourier-Domain (swept-source) system, without the need for considerable changes to the OCT optical module. However, we used our FF-OCT setup to implement ...

      Read Full Article
    12. Retinal Changes in Different Grades of Retinal Artery Occlusion: An Optical Coherence Tomography Study

      Retinal Changes in Different Grades of Retinal Artery Occlusion: An Optical Coherence Tomography Study

      Purpose : To investigate layer-by-layer changes in retinal thickness and reflectivity regarding the severity grade of acute retinal artery occlusion (RAO) using spectral-domain optical coherence tomography (SD-OCT). Methods : This study is a retrospective, observational case-control series that took place in an institutional setting and included 148 eyes from 74 patients diagnosed with acute RAO (central or branch). SD-OCT examinations were taken at baseline. Based on OCT findings, RAO was categorized into three grades (incomplete, subtotal, total). The thickness and reflectivity of selected retinal layers were measured from SD-OCT images. The data were compared across the three grades and against the contralateral ...

      Read Full Article
    13. Optical Coherence Tomography Angiography in the Evaluation of Geographic Atrophy Area Extension

      Optical Coherence Tomography Angiography in the Evaluation of Geographic Atrophy Area Extension

      Purpose : To investigate the application of optical coherence tomography angiography (OCT-A) in evaluation of geographic atrophy (GA) secondary to age-related macular degeneration (AMD). Methods : Patients with GA were prospectively enrolled and studied with blue fundus autofluorescence (FAF), en face structural OCT, and OCT-A. OCT-A images were acquired using a slab of whole choroid, whereas en face structural OCT images were obtained at the ellipsoid zone (EZ), at the choroidal (CH) level, and at the scleral (SC) level. Three readers independently measured the GA extension areas and evaluated the foveal sparing in each examination. Intraobserver/interobserver agreements and agreement between each ...

      Read Full Article
    14. Noninvasive structural and microvascular anatomy of oral mucosae using handheld optical coherence tomography

      Noninvasive structural and microvascular anatomy of oral mucosae using handheld optical coherence tomography

      In this study, we demonstrated the feasibility of using a handheld optical coherence tomography (OCT) for in vivo visualizations of the microstructural and microvascular features of various oral mucosal types. To scan arbitrary locations of the oral mucosa, a scanning probe was developed, composed of a probe body fabricated by a 3D printer, miniaturized two-axis galvanometer, relay lenses, and reflective prism. With a 3D printing technique, the probe weight and the system volume were greatly reduced, enabling the effective improvement of imaging artifacts from unconscious motion and system complexity. Additionally, in our design, the distal end of the probe can ...

      Read Full Article
    15. See all articles
  1. Categories

    1. Applications:

      Art, Cardiology, Dentistry, Dermatology, Developmental Biology, Gastroenterology, Gynecology, Microscopy, NDE/NDT, Neurology, Oncology, Ophthalmology, Other Non-Medical, Otolaryngology, Pulmonology, Urology
    2. Business News:

      Acquisition, Clinical Trials, Funding, Other Business News, Partnership, Patents
    3. Technology:

      Broadband Sources, Probes, Tunable Sources
    4. Miscellaneous:

      Jobs & Studentships, Student Theses, Textbooks
  2. Organizations in the News

    1. (2 articles) Columbia University
    2. (2 articles) University of Washington
    3. (1 articles) Lehigh University
    4. (1 articles) Centre Hospitalier Intercommunal de Créteil
    5. (1 articles) Vanderbilt University
    6. (1 articles) Università Vita-Salute San Raffaele
    7. (1 articles) University of Manitoba
    8. (1 articles) Cornell University
    9. (1 articles) University of Western Australia
    10. (1 articles) Nanyang Technological University
  3. People in the News

    1. (2 articles) Robert Ritch
    2. (2 articles) Donald C. Hood
    3. (2 articles) Jeffrey M. Liebmann
    4. (2 articles) John B. Simpson
    5. (1 articles) Peter B. Noble
    6. (1 articles) Daniël M. de Bruin
    7. (1 articles) David D. Sampson
    8. (1 articles) Rodney W. Kirk
    9. (1 articles) Jiawen Li
    10. (1 articles) Dirk J. Faber
    11. (1 articles) Ton G. van Leeuwen
    12. (1 articles) Brad Fortune
    13. (1 articles) Robert A. McLaughlin
    14. (1 articles) Yuankai K. Tao
    15. (1 articles) Meng-Tsan Tsai
  4. OCT Companies in the News

    1. (2 articles) Optovue
    2. (2 articles) Avinger
    3. (1 articles) Olympus
  5. Picture Gallery

    Advances in intraoperative optical coherence tomography for surgical guidance Postdoctoral Fellow Positions at Case Western Reserve University Postdoctoral Research Fellowship in Biomedical Optics at CNRS/Strasbourg University Investigating the healing mechanisms of an angiogenesis-promoting topical treatment for diabetic wounds using multimodal microscopy Computer-aided detection of early Barrett’s neoplasia using volumetric laser endomicroscopy Feature Of The Week 10/1/2017: Optical Coherence Tomography: from Healthcare Idea to Healthcare Impact. OCT Made Easy (Textbook) 3D Mapping of Choroidal Thickness from OCT B-Scans Multi-layer 3D Simultaneous Retinal OCT Layer Segmentation: Just-Enough Interaction for Routine Clinical Use Optical Coherence Tomography Denoising by Means of a Fourier Butterworth Filter-Based Approach Feature Definition and Selection for Epiretinal Membrane Characterization in Optical Coherence Tomography Images Resin infiltration analysis into carious lesions by optical coherence tomography
  6. Recent Quotes

    1. Optovue continues to lead innovation in Optical Coherence Tomography (OCT) imaging with the first non-contact technique of measuring the epithelial layer of the cornea...Our epi-mapping software has been the subject of more than 200 publications, underscoring its importance and value in the care of patients with corneal and ocular surface conditions. With epi-mapping, physicians are able to quantify epithelial thickness in patients in a manner that is suitable for daily clinical practice.
      By Jay Wei
    2. Combining en-face OCT with movement detection within blood vessels, we get OCT-A, and with it a new imaging era.
      By Eric H. Souied
    3. Obtaining the Category III CPT code for OCT of the skin is an important step towards our goal of obtaining reimbursement and coverage, and we welcome the new codes that can be used by clinicians to record their clinical use of VivoSight OCT in their practice.
      By Jon Holmes
    4. We showed that you can take effectively any OCT system out there and, with minimal changes, boost its resolution to the point where it can detect anatomical features smaller than the size of a typical cell.
      By Adam de la Zerda
    5. This honor highlights the commitment Avinger has to bring the most advanced technology to market for physicians dedicated to treating PAD and their patients who are affected by this disease...Pantheris radically changes the treatment of vascular disease and the Edison Award represents a win for our entire Lumivascular portfolio.
      By John B. Simpson
    6. While we have been actively advancing our lesion assessment technology for the last two years, we are very pleased to see that the significant Series B investment will give MedLumics the means to complete our best-in-class product, conduct a series of international clinical trials to prove its safety and efficacy, and market it to specialist centers. As the company advances and grows into new areas, we will now expand and develop the team internationally.
      By Eduardo Margallo Balbás
    7. It's the most rapidly adopted ophthalmic technology in history.
      By Joel S. Schuman
    8. Our OCT Angiography congresses is a major force in promoting the transition from FA to OCTA by educating the ophthalmologists .
      By Bruno Lumbroso
    9. The development of OCT in the early 1990s greatly benefited from components and methods used in fiber-optical communications...And still, 25 years later, advances in the optical communications industry continue to greatly benefit OCT.
      By James G. Fujimoto
    10. Research by our group at MIT and our collaborators at Praevium Research and Thorlabs indicated that the coherence length of the VCSEL source was orders of magnitude longer than other swept laser technologies suitable for OCT, which suggested the possibility of long-range OCT imaging.
      By Benjamin M. Potsaid